General Stability | Organism |
---|---|
glycerol and ATP required for stabilization | Oryctolagus cuniculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-hydroxyquinoline 5-sulfonate | no inhibition in the presence of excess iron | Oryctolagus cuniculus | |
bathophenanthroline sulfonate | no effect in the presence of excess iron | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, Sephadex G-200, dATP-Sepharose | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bone marrow | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00837 | - |
- |
Oryctolagus cuniculus |
Storage Stability | Organism |
---|---|
-15°C or -70°C, 10 mM histidine-HCl, pH 7.0, 2 mM dithiotreitol, 40% glycerol, 2 mM ATP, 6 months, no loss of activity | Oryctolagus cuniculus |
-15°C, 10 mM histidine-HCl, pH 7.0, 2 mM dithiotreitol, 2 mM ATP, 6 months, 80% loss of activity | Oryctolagus cuniculus |
-70°C, 10 mM histidine-HCl, pH 7.0, 2 mM dithiothreitol, 2 mM ATP, 6 months 60% loss of activity | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ribonucleoside diphosphate + reduced thioredoxin | - |
Oryctolagus cuniculus | 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O | - |
ir |